B2M-associated HLA-G/aAPC Cell
CBP74189
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| I. Background | |
| HLA-G基因是一個(gè)功能性的非經(jīng)典I類HLA分子，具有經(jīng)典樣HLA抗原結(jié)構(gòu)，位于染色體6p21.3的MHC區(qū)，然而與經(jīng)典HLA-I類分子相比，HLA-G具有更少的多態(tài)性和更有限的組織分布。β2-微球蛋白（B2M）是細(xì)胞膜上組織相容性復(fù)合體（MHC，一組編碼人類白細(xì)胞表面抗原HLA基因群的通稱）輕鏈結(jié)構(gòu)的組成部分，在正常細(xì)胞新陳代謝過(guò)程中與MHC分離后釋放入血。β2-微球蛋白（B2M）分布于幾乎所有有核細(xì)胞（尤其在淋巴細(xì)胞和單核細(xì)胞存在豐富）和生物體液，包括血清、尿液和（關(guān)節(jié)）滑液。 | |
| II. Introduction | |
| Host Cell: | N/A |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+3μg/ml puromycin+600μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing B2M. |
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Figure 2. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing HLA-G. |
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Figure 3. Dose Response of LILRB1 Blocking Antibody in LILRB1 Effector Reporter Cells (C13) With B2M-associated HLA-G/aAPC Cells (C15). |
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Figure 4.Dose Response of LILRB2 Blocking Antibody in LILRB2 Effector Reporter Cells (C24) With B2M-associated HLA-G/aAPC Cells(C15). |
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