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HCT116-Luc

CBP30007L

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I. Introduction
Host Cell: HCT116
Expressed gene: Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: McCoy's 5A+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen
 
II. Description of Host Cell Line
Organism: Homo sapiens,human
Tissue: Colon
Disease: Colorectal carcinoma
Morphology: Epitheloid cell
Growth Properties: Adherent
 
III. Representative Data
CBP30007L 信號(hào)

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 530±10(Mean±SD) on HCT116 cells while 12974128±557670(Mean±SD) on HCT116-Luc cells.

 
IV. Stablity Test
CBP30007L 穩(wěn)定性

Figure. Stablity test results. HCT116-Luc cells showed at least 32 passage stability with similar luminescence unit.


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