FcεRI Effector Reporter Cell
CBP74285
詢 價
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |
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IgE由兩條重鏈（ε鏈）和兩條輕鏈組成，F(xiàn)c段含高親和力受體（FcεRI）結(jié)合位點，半衰期僅2-3天（IgG為21天）。作為免疫系統(tǒng)的“哨兵”，IgE既可結(jié)合寄生蟲表面抗原，激活嗜酸性粒細胞釋放毒性顆粒（如主要堿性蛋白），通過ADCC（抗體依賴細胞介導(dǎo)的細胞毒性）清除寄生蟲（如血吸蟲、鉤蟲）；也能因過度反應(yīng)引發(fā)致命過敏；IgE還可靶向腫瘤抗原（如HER2），激活巨噬細胞ADCC效應(yīng)；并且最近有研究指出，腦膜肥大細胞通過IgE-MRGPRX2可加劇阿爾茨海默病病理。 |
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| II. Description | |
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FcεRI Effector Reporter Cell 藥靶模型很好的模擬了 FcεRI的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
Figure 1. FcεRI Effector Reporter Cell細胞模型原理圖 |
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| III. Introduction | |
| Expressed gene: | FcεRI |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+ 1 μg/ml Puromycin+ 10 μg/ml Blasticidin + 800 μg/ml Hygromycin B+ 1000 μg/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant FcεRI Effector Reporter Cell stably expressing FcεRIA.  Figure 3. Dose Response of Human IgE in FcεRI Effector Reporter Cell(C4).  Figure 4. Inhibition of Human IgE-induced Reporter Activity by Human IgE Neutralization Ab in FcεRI Effector Reporter Cell(C4). |
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