NK92（人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞）
CBP60980
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| I. General information | |
| Synonyms: | NK92 |
| Background: | NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. |
| Species: | Homo sapiens, human |
| Tissue: | peripheral blood |
| Disease: | malignant non-Hodgkin's lymphoma |
| Gender: | male, 50 years, Caucasian |
| Morphology: | lymphoblast |
| Growth Mode: | suspension, multicell aggregates |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,12 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 18 Our Cell Line Authentication Service |
| Culture Medium: |
75% MEM α (GIBCO 12561-056 ) + 12.5% FBS+12.5% horse serum + 0.2 mM inositoll+0.02 mM folic acid+200 U/ml recombinant IL-2 NK92完全培養(yǎng)基，# CBP60980M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -, HLA-DR - |
| Comments: | The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92 cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR. For more information, please contact us(4008-750-250). |
