I. General information |
Synonyms: |
CAL-12T |
Background: |
established from a male patient with a non-small cell lung carcinoma in 1981 |
Species: |
human (Homo sapiens) |
Tissue: |
lung |
Disease: |
non-small cell lung carcinoma |
Gender: |
N/A |
Morphology: |
adherent epithelial-like cells growing in monolayers; image; image |
Growth Mode: |
N/A |
Doubling Time: |
ca. 50 hours |
DNA Profile: |
N/A
Our Cell Line Authentication Service |
Culture Medium: |
DMEM+10%FBS
CAL-12T完全培養(yǎng)基,# CBP60050M
We strongly suggest to purchase the complete medium from us.
|
Cryopreservation medium: |
90%FBS+10%DMSO |
Mycoplasma: |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, vimentin - |
Fingerprint: |
multiplex PCR of minisatellite markers revealed a unique DNA profile |
Species: |
confirmed as human with IEF of AST, NP |
Cytogenetics: |
human hypotriploid karyotype with 12% polyploidy - 59(57-59)<3n>X, +1, -2, -4, -5, -6, -6, -7, -11, -12, -13, -16, -16, -18, +20, +4-5mar, der(1;18)(p10;q10), der(1)t(X;1)(?;p?21)t(1;?)(q1?1;?), der(1)(q12->q10::p10->pter), add(3)(p11), add(4)(p11), add(5)(p15), add(9)(q1?)x1-2, add(11)(q24), dup(11)(q13q24), del(12)(p12), i(14q), add(19)(q11), i(21q) |
Virus |
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
Comments: |
For more information, please contact us (4008-750-250). |